Cell-based assays Support Page
One of the most common uses for the In-Cell Western (ICW) assay is to study the effect of a particular compound or compounds, such as drugs, on a signaling pathway.
The first stage of this is to have an idea of the pathway and the possible effect or effects that a chosen compound may have on this pathway.
In this example, we are looking at the EGFR signaling pathway. Specifically, we will look at 3 targets, p-EGFR, p-STAT3 and p-ERK and the effect of 2 drugs, PD168393 (OR compound 1) and U0126 (OR compound 2) on these targets. It is possible to use the ICW to determine the effect of these drugs on these targets of choice.
The first stage of this is to have an idea of the pathway and the possible effect or effects that a chosen compound may have on this pathway.
In this example, we are looking at the EGFR signaling pathway. Specifically, we will look at 3 targets, p-EGFR, p-STAT3 and p-ERK and the effect of 2 drugs, PD168393 (OR compound 1) and U0126 (OR compound 2) on these targets. It is possible to use the ICW to determine the effect of these drugs on these targets of choice.
Publications
- Quantitative methods for the analysis of protein phosphorylation in drug development
- Epithelial Cell Assays for the Odyssey System
- A High Throughput Drug Discovery for Intracellular Neurodegenerative Disease-Associated Targets using the LI-COR Odyssey Near Infrared System
- Tracking G Protein-coupled Receptor Trafficking Using Odyssey Imaging.
No comments:
Post a Comment